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School of Molecular & Biomedical Science
The University of Adelaide
AUSTRALIA 5005

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Dr David Ellis
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Mould Identification: A Virtual Self Assessment

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Please find additional information below

Unknown 45 = Phialophora richardsiae

Case History: A 25-year-old male renal transplant recipent presented with a dark coloured subcutaneous nodule on his leg, which later became ulcerated. The culture shown below was isolated.

Culture:

Culture

Colonies grow rapidly, and are powdery to woolly or tufted, greyish-brown with a grey-brown to olivaceous-black reverse.

Microscopy:

Two conidial types are produced; (1) hyaline conidia which are allantoid or cylindrical, 3-6 x 1.5-2.5 μm in size, formed on inconspicuous, peg-like phialides on thin-walled hyphae; and (2) brown, thick-walled conidia which are spherical to sub-spherical, 2.5-3.5 x 2-3 μm, formed on dark brown, slender, tapering phialides with flaring collarettes. RG-2 organism.

Microscopy

Microscopy

Microscopy

Phialides of P. richardsiae producing 2 types of conidia. (1) hyaline conidia, formed on inconspicuous, peg-like phialides on thin-walled hyphae; and (2) brown, thick-walled conidia formed on dark brown, slender, tapering phialides with flaring collarettes.

MIC data is limited.  Antifungal susceptibility testing of individual strains is recommended.

Antifungal
MIC ug/mL
Antifungal
MIC ug/mL
Antifungal
MIC ug/mL
Range
Range
Range
Amphotericin B
0.125-1
Itraconazole
0.03-2
Voriconazole
0.125-2

Comment:
Phialophora richardsiae is a soft rot fungus of  wood and is an uncommon cause of human infection.  However, cases of subcutaneous phaeohyphomycosis have been reported.

For further information on Phaeohyphomycosis.

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What is your identification?

Acrophialophora fusispora
Phialophora richardsiae
Phialophora verrucosa

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