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Fungal Descriptions

Laboratory Methods

	Antifungal Susceptibility Testing

	Culture Techniques and Media

	Microscopy Techniques and Stains

	Specimen Collection and Processing

School of Molecular & Biomedical Science
THE UNIVERSITY OF ADELAIDE
AUSTRALIA 5005

Contact:
Dr David Ellis

Email

Telephone:
+61 8 8161 6459
Facsimile:
+61 8 8161 7589

Lactophenol Cotton Blue.

For the staining and microscopic identification of fungi

Cotton Blue (Aniline Blue)	0.05 g
Phenol Crystals (C6H5O4)	20 g
Glycerol	40 ml
Lactic acid (CH3CHOH COOH)	20 ml
Distilled water	20 ml

Method

This stain is prepared over two days.

1. On the first day, dissolve the Cotton Blue in the distilled water. Leave overnight to eliminate insoluble dye.

2. On the second day, wearing gloves add the phenol crystals to the lactic acid in a glass beaker. place on magnetic stirrer until the phenol is dissolved.

3. Add the glycerol.

4. Filter the Cotton Blue and distilled water solution into the phenol/glycerol/lactic acid solution. Mix and store at room temperature.