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Fungal Descriptions

Laboratory Methods

	Antifungal Susceptibility Testing

	Culture Techniques and Media

	Microscopy Techniques and Stains

	Specimen Collection and Processing

School of Molecular & Biomedical Science
THE UNIVERSITY OF ADELAIDE
AUSTRALIA 5005

Contact:
Dr David Ellis

Email

Telephone:
+61 8 8161 6459
Facsimile:
+61 8 8161 7589

Cellotape Flag Preparations.

An excellent technique for the rapid mounting of sporulating fungi because it keeps more of the reproductive structures intact.

1. Using clear 2cm wide cellotape and a wooden applicator stick (orange stick) make a small cellotape flag (2x2cm).

2. Using sterile technique, gently press the sticky side of the flag onto the surface of the culture.

3. Remove and apply a drop of 95% alcohol to the flag, this acts as a wetting agent and also dissolves the adhesive glue holding the flag to the applicator stick.

4. Place the flag onto a small drop of Lactophenol cotton blue on a clean glass slide, remove the applicator stick and discard, add another drop of stain, cover with a coverslip, gently press and mop up any excess stain