Urease Agar with 0.5% Glucose

Ingredients:

Urea, broth base (Oxoid CM71) 0.9 g

Distilled water 91 ml

Bacto Agar (BD) 1.5 g

Method:

1. Place ingredients into 100ml bottle.

2. Autoclave at 115C for 20 minutes.

3. Cool to 50C.

4. Add aseptically 5ml of sterile urea solution (Oxoid SR20).

5. All 4ml of 10% Sterile Glucose solution.

6. Dispense for slopes, and slope on rack.

7. Incubate overnight.