Sabouraud's Dextrose Agar with 5% NACL

For the cultivation and differentiation of dermatophytes especially T. rubrum from T. mentagrophytes.

Ingredients:

Sabouraud Dextrose Agar (Oxoid CM41) 32.5 g

Sodium Chloride NaCl 25 g

Distilled water 500 ml

Method:

1. Soak ingredients in approximately 100ml water.

2. Bring remaining water to boil, add to soaking ingredients.

3. Dispense for slopes.

4. Autoclave at 118C for 10 minutes, then slope on racks.