Lactrimel Agar for dermatophytes
For the production of pigment by Trichophyton rubrum
Ingredients:
| Skimmed milk powder (use only Dutch Jug skimmed milk powder). | 7 g |
| Honey | 10 g |
| Cornmeal agar (BD) | 17 g |
| Chloramphenicol | 1x250 mg capsule |
| Distilled water | 1000 ml |
Method:
1. Weigh skimmed milk into stainless steel jug. Slowly add some water, mixing milk into smooth paste. Continue adding small quantities of water until powder is dissolved (about 150ml).
2. Weigh other ingredients into skimmed milk and allow to soak.
3. Boil remaining water, and with it wash out honey from beaker.
4. Add to other ingredients and boil.
5. Dispense for slopes if required.
6. Autoclave for 10 minutes at 115oC.
7. On removal from autoclave allow to stand 5 minutes then shake and slope on racks or pour plates which ever is applicable.
Note: Do not filter or adjust pH in any way
