Brain Heart Infusion Agar (BHIA) with 5% Sheep Blood
For the primary isolation and cultivation of yeasts and moulds.
Ingredients:
| Brain-heart infusion agar (Oxoid) | 52 g |
| Sheep blood | 50 ml |
| Gentamicin (40 mg/ml) | 0.65 ml |
| Chloramphenicol | 1x250 mg capsule |
| Distilled water | 1000 ml |
Method:
1. Soak BHI and antibiotics in 100 ml water.
2. Boil remaining water, add to soaking ingredients, and bring to boil to dissolve, stirring well to prevent from charring.
3. Autoclave at 121C for 15 minutes, and aseptically add the blood to the cooled (50C) medium before dispensing for slopes, or for plates as required.
