Vitamin Free Agar (Trichophyton Agar No 1).

For the differentiation of Trichophyton species

Ingredients:

Trichophyton Agar No. 1 (BD)  11.8 g

Distilled water 200 ml

Method:

1. Add agar to water in stainless steel jug, allow to soak.

2. Bring to boil to dissolve, stirring constantly.

3. Once boiled remove immediately to avoid discolouration.

4. Dispense for slopes.

5. Autoclave at 118C for 10 minutes, remove and slope.