You are here: 
text zoom : S | M | L
Printer Friendly Version
Further Enquiries

School of Biological Sciences
The University of Adelaide
AUSTRALIA 5005

Contact:
Dr David Ellis
Email


Trematosphaeria grisea

Synonymy: Madurella grisea

RG-2 organism.

Morphological Description: Colonies are slow growing, dark, leathery, folded with radial grooves and with a light brown to greyish surface mycelium. With age, colonies become dark brown to reddish-brown and have a brownish-black reverse. Microscopically, cultures are sterile, although hyphae of two widths have been described, thin at 1-3 µm in width or broad at 3-5 µm in width. The optimum temperature for growth of T. grisea is 30C; this fungus does not grow at 37C.

Trematosphaeria grisea can be distinguished from Madurella mycetomatis by the inability to grow at 37C and to assimilate lactose.

Key Features: Black grain mycetoma, no growth at 37OC, no diffusible brown pigment produced on culture and absence of conidia.

References: McGinnis (1980), Chandler et al. (1980), Rippon (1988), de Hoog et al. (2000, 2015), Ahmed et al. (2014b), Desnos-Olliver et al. (2006), Irinyi et al. (2015).

Grains of tissue microcolonies are black

Trematosphaeria grisea grains (tissue microcolonies) are black, round to lobed, soft to firm, up to 1.0 mm, with two distinctive zones, a hyaline to weakly pigmented central zone and a deeply pigmented periphery.

MIC data is limited.  Antifungal susceptibility testing of individual strains is recommended.

Antifungal
MIC ug/mL
Antifungal
MIC ug/mL
Antifungal
MIC ug/mL
Range
Range
Range
Amphotericin B
0.25
Itraconazole
0.5
Voriconazole
0.5

 

Mycosis: Mycetoma